Fig. 3

RNA-seq analysis unravels ZEN-induced toxicity on the immune system. a–f ZEN disturbs the immune system (e.g., liver, spleen, thymus, and inguinal lymph nodes) in pigs, during phase-1/2. a Number of DEGs detected in immune organs. b Statistical analysis of top30 KEGG pathways enriched by DEGs in immune organs. c Venn map of KEGG pathways related to immune disease and system in immune organs. d Number of overlapping KEGG pathways between phase-1 and phase-2 pigs and its corresponding number of common genes in overlapping pathways. e Other top30 KEGG pathways enriched by DEGs in immune organs. f Representative Western blots and its quantifications of key proteins involved in NF-κB (e.g., p-p65), CAMs (e.g., CD54/ICAM-1 and E-cadherin), and phagosome (e.g., CD63 and TLR4) pathways (n=4). Bar values are means ± SEM. *P < 0.05