Target/purpose | Sequence | Source |
---|---|---|
Primers for mutagenesis | ||
 Deletion of E. coli MP1 lldD | 5′-GCTTCTTCTAGAGGTACCGCATGGTGCTGCTCAGTCGACGTG-3′ 5′-CCCTTAAGCTTCATGCGTTTTTCTCCCTCG-3′ 5′- AAACGCATGAAGCTTAAGGGTTAGACGAATATC-3′ 5′-GGAGAGCTCGATATCGCATGTATGGTGATGGGATCTGAC-3′ | This study |
 Confirmation of E. coli MP1 lldD deletion | 5′-TGTCAGACGAGGTTGCC-3′ 5′-CAATCTGTGACGCTTGGC-3′ | This study |
 Deletion of E. coli MP1 dld | 5′-GCTTCTTCTAGAGGTACCGCATGCGACTGTTTTCACCGCATC-3′ 5′-TCCGTTATTATGTGGTGGCGAAAAAAATATC-3′ 5′-CGCCACCACATAATAACGGATGGCAGAG-3′ 5′-GGAGAGCTCGATATCGCATGCGCTGATGTCTTCAGAAAAC-3′ | This study |
 Confirmation of E. coli MP1 dld deletion | 5′-AAGCAGAGACACGCCCG-3′ 5′-AACAGCGAAATCAGCCG-3′ | This study |
 Deletion of E. coli MP1 ykgEFG | 5′-GCTTCTTCTAGAGGTACCGCATGGTGAATCATCTTTTCACAAG-3′ 5′-CATATCTCTCACTTCATGCCCATTTATG-3′ 5′-GGCATGAAGTGAGAGATATGTAGTCTGGAC-3′ 5′-GGAGAGCTCGATATCGCATGATGGTCAGGAGATAAGAG-3′ | This study |
 Confirmation of E. coli MP1 ykgEFG deletion | 5′-ATCGCTGAGTCAGTAGGC-3′ 5′-ATCAGACAACACCAGGC-3′ | This study |
 Deletion of EcN lldD | 5′-GCTTCTTCTAGAGGTACCGCATGGCCGATGATCCGGATTAC-3′ 5′-CGTCTAACCCGCGTTTTTCTCCCTCGAATG-3′ 5′-AGAAAAACGCGGGTTAGACGAATATCTGCTATCCTGC-3′ 5′-GGAGAGCTCGATATCGCATGCGGATACCCCAGCTGGCG-3′ | This study |
 Confirmation of EcN lldD deletion | 5′-AGGTGTGCTGCTCAGTCGAC-3′ 5′-GTGAAGTGGTGGAAGAAGCC-3′ | This study |
qPCR primers for Mus Musculus | ||
 Nos2 | 5′-TTGGGTCTTGTTCACTCCACGG-3′ 5′-CCTCTTTCAGGTCACTTTGGTAGG-3′ | [43] |
 Tnf | 5′-AGCCAGGAGGGAGAACAGAAAC-3′ 5′-CCAGTGAGTGAAAGGGACAGAACC-3′ | [34] |
 Ifng | 5′-TCAAGTGGCATAGATGTGGAAGAA-3′ 5′-TGGCTCTGCAGGATTTTCATG-3′ | [44] |
 Gapdh | 5′-TGTAGACCATGTAGTTGAGGTCA-3′ 5′-AGGTCGGTGTGAACGGATTTG-3′ | [44] |