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Table 2 Oligonucleotides used in this study

From: Colonocyte-derived lactate promotes E. coli fitness in the context of inflammation-associated gut microbiota dysbiosis

Target/purpose

Sequence

Source

Primers for mutagenesis

 Deletion of E. coli MP1 lldD

5′-GCTTCTTCTAGAGGTACCGCATGGTGCTGCTCAGTCGACGTG-3′

5′-CCCTTAAGCTTCATGCGTTTTTCTCCCTCG-3′

5′- AAACGCATGAAGCTTAAGGGTTAGACGAATATC-3′

5′-GGAGAGCTCGATATCGCATGTATGGTGATGGGATCTGAC-3′

This study

 Confirmation of E. coli MP1 lldD deletion

5′-TGTCAGACGAGGTTGCC-3′

5′-CAATCTGTGACGCTTGGC-3′

This study

 Deletion of E. coli MP1 dld

5′-GCTTCTTCTAGAGGTACCGCATGCGACTGTTTTCACCGCATC-3′

5′-TCCGTTATTATGTGGTGGCGAAAAAAATATC-3′

5′-CGCCACCACATAATAACGGATGGCAGAG-3′

5′-GGAGAGCTCGATATCGCATGCGCTGATGTCTTCAGAAAAC-3′

This study

 Confirmation of E. coli MP1 dld deletion

5′-AAGCAGAGACACGCCCG-3′

5′-AACAGCGAAATCAGCCG-3′

This study

 Deletion of E. coli MP1 ykgEFG

5′-GCTTCTTCTAGAGGTACCGCATGGTGAATCATCTTTTCACAAG-3′

5′-CATATCTCTCACTTCATGCCCATTTATG-3′

5′-GGCATGAAGTGAGAGATATGTAGTCTGGAC-3′

5′-GGAGAGCTCGATATCGCATGATGGTCAGGAGATAAGAG-3′

This study

 Confirmation of E. coli MP1 ykgEFG deletion

5′-ATCGCTGAGTCAGTAGGC-3′

5′-ATCAGACAACACCAGGC-3′

This study

 Deletion of EcN lldD

5′-GCTTCTTCTAGAGGTACCGCATGGCCGATGATCCGGATTAC-3′

5′-CGTCTAACCCGCGTTTTTCTCCCTCGAATG-3′

5′-AGAAAAACGCGGGTTAGACGAATATCTGCTATCCTGC-3′

5′-GGAGAGCTCGATATCGCATGCGGATACCCCAGCTGGCG-3′

This study

 Confirmation of EcN lldD deletion

5′-AGGTGTGCTGCTCAGTCGAC-3′

5′-GTGAAGTGGTGGAAGAAGCC-3′

This study

qPCR primers for Mus Musculus

 Nos2

5′-TTGGGTCTTGTTCACTCCACGG-3′

5′-CCTCTTTCAGGTCACTTTGGTAGG-3′

[43]

 Tnf

5′-AGCCAGGAGGGAGAACAGAAAC-3′

5′-CCAGTGAGTGAAAGGGACAGAACC-3′

[34]

 Ifng

5′-TCAAGTGGCATAGATGTGGAAGAA-3′

5′-TGGCTCTGCAGGATTTTCATG-3′

[44]

 Gapdh

5′-TGTAGACCATGTAGTTGAGGTCA-3′

5′-AGGTCGGTGTGAACGGATTTG-3′

[44]