Fig. 6

Salmonella consumes lactate generated by the intestinal epithelium. A–D Streptomycin-treated wild-type mice were infected with the S. Tm wild-type strain for 5 days. Mock-treated animals were used as controls. Butyrate (A) and lactate (B) concentrations in the colon content. Butyrate (C) and lactate (D) concentrations in the cecal content. E–G Wild-type mice (wt), Ldha^ΔIEC mice (Ldha^ΔIEC), or Ldha^fl/fl (Ctrl) littermates were either mock-infected (mock) or infected with a 1:1 mixture of S. Tm AJB715 (WT) and the ΔlldD mutant for 5 days. E mRNA levels of iNOS (Nos2) and IFN-g (Ifng) in the cecal tissue, as determined by RT-qPCR. mRNA levels are normalized to Gapdh mRNA and represent the fold change over mock-treated wild-type mice. F Competitive index in the cecal content. G Competitive Index in the colon content. Columns represent geometric mean and error bars represent geometric standard deviation. Each dot represents one animal. Statistical significance was determined using a two-way ANOVA with Sidak’s multiple comparisons test. **, p<0.01; ns, not statically significant