Fig. 6

Chromosome observation of the long filamentous bacteria using YOYO™-1 labeling. DNA (in green) was specifically labeled using the YOYO™-1 dye. The host nucleus is not visible here as being at the opposite of the microvilli of the epithelial host tissue (t). A Long filamentous bacteria (b) along the midgut brush-border cells. The white square is image B with a closer view. The scale bar represents 2 μm. B YOYO™-1 labeling reveals numerous bacterial chromosomes per cell (green balls). Cells are non-segmented and white arrows point out each chromosome. The scale bar represents 0.5 μm. C Fluorescence intensity profile within a bacterial cell revealing that chromosomes are regularly spaced by 0.6 to 0.7 μm