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Fig. 6 | Microbiome

Fig. 6

From: Tick transmission of Borrelia burgdorferi to the murine host is not influenced by environmentally acquired midgut microbiota

Fig. 6

Impairing key regulators of the immune deficiency pathway (IMD) influences spirochete growth but not migration in the tick. A cocktail of short interfering (si) RNA targeting key components of the IMD pathway, XIAP and p47 (ds xiap/p47), or control ds gfp RNA (ds gfp) was injected into the anal pore of B. burgdorferi-infected nymphs and ticks fed to repletion on pathogen-free C3H/HeN mice and the following parameters evaluated: A Engorgement weights; qRT-PCR assessment of transcript levels for B p47 in tick midguts; C xiap in tick midguts; qPCR assessment of DB. burgdorferi-specific flagellin B (flaB) in tick midguts and salivary glands (sg); E 16S rRNA as a proxy for total bacterial burden; FRickettsia buchneri (R. buchneri)-specific 16S rRNA; GStaphylococcus genera-specific 16S rRNA; HPseudomonas genera-specific 16S rRNA. (sg); Each data point in A represents 1 tick; B–H represent a pool of 3 ticks; Error bars are + SEM. Significance of differences assessed by non-parametric Mann-Whitney test (***p<0.0001; *p<0.05)

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