Fig. 1
From: Low-abundance populations distinguish microbiome performance in plant cell wall deconstruction
Experimental overview. Green waste compost samples were collected and used to inoculate M9TE media mixed with sorghum biomass and samples were incubated for 8 weeks (Tier 1). After 56 days of incubation, each of the three communities from Tier 1 (comm1, comm2, comm3) were used to inoculate a second series of flasks (Tier 2) with either wild-type (WT) or stacked bmr6 × bmr12 mutant (SM) sorghum. Tier 2 samples were incubated for 2 weeks. Changes in biomass content, enzymatic activities, lignin content, and bacterial community dynamics and activity were assessed using the suit of tools depicted in the image. MAGs metagenome-assembled genomes, NIMS nanostructure-initiator mass spectrometry, DNS dinitrosalicylic acid, ABSL acetyl bromide soluble lignin