Fig. 5

a Proportion of probe-covered bases that received TELSeq read coverage of at least 1× (on-target, green line), versus proportion of non-probe-covered bases that received TELSeq read coverage of at least 1× (off-target, black line). b Proportion of probe-covered bases for which high-depth GridION (gray line) and ultra high-depth PromethION (purple line) read coverage exceeded that of TELSeq coverage. Parentheses in figure legend indicate the relative sequencing throughput difference of GridION and PromethION platforms relative to TELSeq, i.e., 5× and 15×. Organisms in MOCK are listed in order of ascending relative abundance, from left to right on the x-axis. L. fermentum, C. neoformans, and S. cerevisiae did not have any known ARGs and did not receive any probe-specific coverage; therefore, the on-target rates by TELSeq (a) and Nanopore are not calculable. L. fermentum, C. neoformans, and S. cerevisiae received 17,922, 1242, and 571 alignments by TELSeq respectively, which can be considered off-target alignments. However, none of these off-target reads aligned to any MEGARes accessions (i.e., false positive ARGs) across any of the MOCK replicates