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Fig. 3 | Microbiome

Fig. 3

From: LotuS2: an ultrafast and highly accurate tool for amplicon sequencing analysis

Fig. 3

Reproducibility from different amplicons sequence data analysis pipelines. Three independent datasets were used to represent different biomes and amplicon technologies, using A, D human faecal samples (16S rRNA gene, N = 40 replicates). B, E soil samples (16S rRNA gene, N = 50 replicates), and C, F soil samples (ITS 2, N = 50 replicates). AC Bray-Curtis distances among technical replicate samples were used to assess the reproducibility of community compositions by different pipelines. The pipeline with the lowest BCd in each subfigure is denoted with a star (*). The significance of pairwise comparisons of each pipeline was calculated using the Tukey’s HSD test (Supplementary Table S2). DF Further, the fraction of technical replicates being closest to each other (BCd) was calculated to simulate identifying technical replicates without additional knowledge. Numbers above bars are the ordered pipelines performing best. Lower Bray-Curtis distances between technical replicates and a higher fraction of correct technical replicates indicate better reproducibility. LotuS2 runs are labelled with red color

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