Fig. 3

Representative CSGs are depicted on the alphaproteobacterial clade of the GTDB-Tk-inferred phylogeny. CSGs are depicted with other genes found in the eukaryotic steroid biosynthesis pathway (PATH:ko00100) that were commonly found flanking the three core genes (TM7SF2/ERG24, CYP51, and LSS/ERG7) and DFR (dihydroflavonol-4-reductase). “Others” refers to the genes not found in pathway ko00100. If no operon is shown for a given MAG, then a CSG was not detected. Arrows denote the direction of the coding sequence. Genes that overlap with the “steroid biosynthesis” panels of Fig. 2 are colored to match. Tree plotting and annotation were performed with the R packages ggtree [51]