Fig. 5

ErbB4 is downregulated in ERβ^−/− mice with experimental colitis. A Hierarchical clustering heatmap of gene expression profile in hypothalamus of WT and ERβ^−/− mice under homeostasis conditions and DSS treatment. n = 3/group. B Venn diagram of differentially expressed genes (DEGs) between control ERβ^−/− and DSS ERβ^−/− mice, and between DSS ERβ^−/− and DSS WT mice. n = 3/group. C Signaling pathway enrichment analysis was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG). Top 10 significantly enriched pathways in the hypothalamus related to the 934 overlapping genes plotted by enrichment score. D Hierarchical clustering heatmap of gene expression profile of ErbB signaling pathway. n = 3/group. E Quantitative real-time PCR analysis of mRNA expressions of ErbB4 and its several downstream genes (Pik3ca, Pik3r1, Akt2, Gsk3b, and Cdkn1a) in the hypothalamus. n = 3/group. F, G Representative images (F) and quantitative analysis (G) of ErbB4 immunofluorescence staining within the paraventricular nucleus (PVN) of WT and ERβ^−/− mice in the baseline state and 5 days after onset of DSS exposure. Scale bar = 50 μm. n = 5/group. Data are presented as mean ± SEM. Statistical comparisons were performed using two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001