Table 1 Primers used for qRT-PCR
Gene | Primer sequence (5′–3′) | |
|---|---|---|
Forward primer | Reverse primer | |
MDA5 | F: TCAGGAGGAGGACGACCACGAT | R: TTCCCACGACTCTCAATAACAG |
OASL | F: CTTGACAGTGGAGAGGG | R:ACGAAGACCTGGATCTCGGA |
Mx | F:AGGGGCCATCACATTCACAT | R:AGATACTTCAGGGGATTCTC |
IFITM5 | F:AAGGTGTCGGAGGATGGTGGTC | R: GGAATCAGCCGCTTGAGACGAG |
IFN-β | F:GCCCACACACTCCAAAACACTG | R: TTGATGCTGAGGTGAGCGTTG |
IBV-N | F: CAAGCTAGGTTTAAGCCAGGT | R: TCTGAAAACCGTAGCGGATAT |
β-actin | F: ATTGCTGCGCTCGTTGTT | R:ATCGTACTCCTGCTTGCTGATCC |