Fig. 3

Innate immune responses were diminished in ABX chicken post-IBV infection. a Frequency of a panel of macrophages in PBMC of mock or infected SPF or ABX chicken (n = 6) at 5 dpi. b Expression of MHC-II on PBMC macrophages from mock or infected SPF or ABX chicken (n = 6) at 5 dpi. c Depletion of splenic macrophages following administration of clodronate liposomes. Histograms showing KUL01+ cells isolated from the spleen of chicken (n = 6) treated with PBS liposomes or clodronate liposomes. d Blood and kidney viral titers of SPF or ABX chicken (n = 6) treated with PBS liposomes or clodronate liposomes at 5 dpi. Data are from three independent experiments. Biological replicates for each sample are three. P values were determined by an unpaired two-tailed Student’s t test. ns, not significant ; **P < 0.01