Fig. 4

RhoB deficiency regulates Wnt and p38 MAPK signaling pathway. A–F RNAseq analysis of IECs from WT and RhoB^−/− mice. n = 5 for each genotype. A Volcano plot showing the upregulated and downregulated genes in IECs from RhoB^−/− mice compared with WT mice. Green indicates downregulated genes; red indicates upregulated genes; gray indicates non-differentially expressed genes (No-DEGS). B KEGG pathway analysis showing distinct differences in pathways between WT and RhoB^−/− mice. C Heatmap of Wnt signaling pathway-associated genes. D GSEA analysis of Wnt signaling pathway-associated genes in WT and RhoB^−/− mice. Normalized enrichment score (NES) and false discovery rates (FDR) are indicated. E Heatmap of goblet cell signature genes. F Heatmap of epithelial cells proliferation-associated genes. G Confocal images of Muc2 and Ki67 staining in colonic organoids of the indicated genotypes. Muc2 or Ki67: red; DAPI: blue (n = 3 from 3 independent experiments). Scale bar: 100 μm. H β-catenin and p-p38 staining in colonic tissues of the indicated genotypes (n = 9 from 3 independent experiments). Scale bar: 50 μm. Data are the mean ± SD. One-way ANOVA (G) or unpaired Student’s t test (H). **p < 0.01, ***p < 0.001, ****p < 0.0001