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Fig. 3 | Microbiome

Fig. 3

From: RhoB affects colitis through modulating cell signaling and intestinal microbiome

Fig. 3

RhoB deficiency promotes intestinal homeostasis. A Alcian blue-Periodic acid Schiff (AB-PAS; indicating goblet cells) staining in colonic tissues of the indicated genotypes. Scale bar: 50 μm. B Muc2 staining in colonic tissues of the indicated genotypes. Scale bar: 50 μm. C Representative confocal images of Muc2 staining (green) and DAPI (blue) in colonic tissues of the indicated genotypes. Quantification of the fluorescence density and inner mucus layer thickness measured from the top of the villi to the periphery of the Muc2-positive region. Scale bar: 10 μm. D Ki67 staining in colon sections of the indicated genotypes. Scale bar: 50 μm. E Bright-field images of colonic organoids and quantitation. Colonic organoids derived from the WT or RhoB+/− and RhoB−/− mice. After 10 days of culturing, the number of organoids per well was counted. Scale bars: 100 μm. F Confocal images of Muc2 and Ki67 staining and quantitation in colonic organoids of the indicated genotypes. Muc2 or Ki67: red; DAPI: blue. Scale bar: 10 μm. n = 9 from 3 independent experiments (A–D), n = 4 from 4 independent experiments (E–F). Data are the mean ± SD. One-way ANOVA (A–F). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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