Fig. 2

RhoB deficiency protects mice from DSS-induced colitis. A–G Eight-week-old male mice were given 1.5% DSS in drinking water for 7 days (n = 12 from 3 independent experiments). A Body weight loss of WT and RhoB^+/−/RhoB^−/− mice after DSS treatment. B Diarrhea scores of WT and RhoB^+/−/RhoB^−/− mice after DSS treatment. C Rectal bleeding scores of WT and RhoB^+/−/RhoB^−/− mice after DSS treatment. D Disease activity index of WT and RhoB^+/−/RhoB^−/− mice after DSS treatment. E Measurement and quantification of colon length in WT and RhoB^+/−/RhoB^−/− mice after DSS treatment. F Representative H&E staining analysis of histopathological changes and quantitation of histology score in colon from DSS-exposed mice of the indicated genotypes. G Alcian blue-Periodic acid Schiff (AB-PAS; indicating goblet cells) staining in colon from DSS-exposed mice of the indicated genotypes. Scale bar: 50 μm. H Fluorescein isothiocyanate (FITC)-dextran measurement in serum from DSS-exposed mice of the indicated genotypes (n = 9 from 3 independent experiments). Data are the mean ± SD. Statistical significance was determined by two-way ANOVA (A–D) or one-way ANOVA (E–H). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001