Fig. 7

Multiple types of markers for discriminating the resistant family from the susceptible family in C. semilaevis. A Comparison of area under receiver operating characteristic curves (AUC), microbial markers (purple curve), functional markers (green curve), and combinatorial markers (red curve). B The AUC, F1 score, and accuracy of microbial markers, functional markers, and combinatorial markers. Four intestinal microbes: Alicyclobacillus, uc_Propionibacteriaceae, Phaeobacter, and Propionibacterium were selected as microbial markers due to their outstanding performance. The functional markers were the host DEGs (|log₂(FoldChange)| > 1 and adjusted p < 0.05) which were significantly correlated with microbes (microbes: relative abundance ≥ 0.1% and coverage > 10% samples; correlation: |Spearman rank correlation| ≥ 0.5 and adjusted p < 0.05). These DEGs were mapped to 11 KO genes (soat, meso1, hsd17b3, cyp27a1, acot1_2_4, cyp2b, cyp19a, irf3, rpc11, elovl5, and akt), which were listed in C. C The correlations between multiple markers and growth characters (body weight and length). Only the Spearman rank correlations with p < 0.05 were considered as significant ones and indicated by “*”