Strain-level resolution and pneumococcal carriage dynamics by single-molecule real-time (SMRT) sequencing of the plyNCR marker: a longitudinal study in Swiss infants

Table 1 Primers used in this study

Gene	Primer	Sequence (5′-3′)	Reference
lytA	Forward	ACGCAATCTAGCAGATGAAGCA	[22]
	Reverse	TCGTGCGTTTTAATTCCAGCT
	Probe	FAM-GCCGAAAACGCTTGATACAGGGAG-BHQ1
plyNCR^a	Forward	[GCAGTCGAACATGTAGCTGACTCAGGTCAC]AAAGGCTGCACGGACATTG	[12]
plyNCR^a	Reverse	[TGGATCACTTGTGCAAGCATCACATCGTAG]CCGATTTGCCACTAGTGCGTAAGC	[12]
cpsA	Forward	GCAGTACAGCAGTTTGTTGGACTGACC	[24]
cpsA	Reverse	GAATATTTTCATTATCAGTCCCAGTC	[24]

^aPacbio Universal Sequence overhang in []. Primers were HPLC purified and blocked at the 5′ site with an amino group

ISSN: 2049-2618