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Fig. 4 | Microbiome

Fig. 4

From: Strain-level resolution and pneumococcal carriage dynamics by single-molecule real-time (SMRT) sequencing of the plyNCR marker: a longitudinal study in Swiss infants

Fig. 4

Sensitivity of the plyNCR for pneumococcal detection in sequenced mock samples. The plyNCR was amplified from 10 pneumococcal mock communities (MC) and sequenced in technical quadruplicates by SMRT sequencing. The top and bottom panels show the abundance of all strains recovered as amplicon sequence variants (ASVs) by DADA2 in MC1-5 (group 1) and MC6-10 (group 2), respectively. MC1, 2, 6, and 7 represent single strain communities. MC3, 4, and 5 contain mixtures of MC1 and 2 in ratios 1:9, 1:49, and 1:89, respectively, while MC8, 9, and 10 contain mixtures of MC6 and MC7 in ratios 1:99, 1:119, and 1:139, respectively. “Two SNPs off” indicates that the least abundant strain in MC5 (1:89) was recovered with all 17 expected SNPs and two additional, erroneous SNPs

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Microbiome

ISSN: 2049-2618

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