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Table 2 The most abundant (> 1%) archaeal and bacterial genera identified in metagenomics and metatranscriptomics data in cows treated with 3-nitrooxypropanol (3-NOP) compared to control cows at weeks 4, 8, and 12 weeks

From: The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows

Genus Control 3-NOP Significance
W4 W8 W12 SEM W4 W8 W12 SEM Trt W4 vs W8 W4 vs W12 Trt:W4 vs W8 Trt:
Trt:W4 vs W12
Archaea-metagenomics
Methanobrevibacter 82.36 75.27 70.38 2.28 73.89 66.96 69.74 2.39 0.02 < 0.001 < 0.001 0.55 < 0.001
Methanobacteriaceae 3.76 5.84 5.52 0.33 4.35 6.61 6.39 0.35 0.02 < 0.001 < 0.001 0.74 0.34
Methanosphaera 3.17 4.81 5.26 0.48 4.41 5.63 5.31 0.46 0.13 < 0.001 < 0.001 0.006 < 0.001
Methanobacterium 0.87 1.39 1.38 0.11 1.21 1.84 1.73 0.13 0.03 < 0.001 < 0.001 0.82 0.27
Methanosarcina 0.9 1.11 1.58 0.16 1.54 1.65 1.44 0.19 0.02 < 0.001 < 0.001 0.65 < 0.001
Thermoplasmata 0.79 1.04 1.3 0.17 1.01 1.59 1.56 0.16 0.01 < 0.001 < 0.001 0.82 < 0.001
Archaea-metatranscriptomics
Methanobrevibacter 78.6 72.19 69.46 1.66 74.01 67.18 66.82 1.84 0.12 < 0.001 < 0.001 < 0.001 < 0.001
Methanosphaera 6.14 9.65 11.07 0.88 7.32 10.68 7.92 0.81 0.76 < 0.001 < 0.001 0.002 < 0.001
Methanobacteriaceae 4.24 6.98 6.86 0.46 6.47 7.24 8.07 0.45 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001
Thermoplasmata 4.83 4.14 4.51 0.39 5.13 6.82 8.03 0.8 0.17 < 0.001 < 0.001 < 0.001 < 0.001
Bacteria- metagenomics
Bacteroidetes Prevotella 16.19 16.14 18.56 0.826 17.54 16.52 14.66 0.81 0.24 < 0.001 < 0.001 < 0.001 < 0.001
Bacteria Unclassified 4.29 4.45 4.32 0.094 4.17 4.41 4.57 0.092 0.40 < 0.001 0.004 < 0.001 < 0.001
Fibrobacteres Fibrobacter 3.16 3.07 3.27 0.346 3.32 2.41 3.64 0.309 0.97 < 0.001 0.36 < 0.001 < 0.001
Firmicutes Butyrivibrio 3.6 3.33 2.72 0.332 2.72 2.85 3.18 0.209 0.006 < 0.001 < 0.001 < 0.001 < 0.001
Bacteroidetes Bacteroides 2.55 2.52 2.75 0.092 3 2.75 2.54 0.094 0.002 0.66 < 0.001 < 0.001 < 0.001
Firmicutes Lachnospiraceae 2.06 2.13 1.94 0.068 1.97 2.02 2.07 0.03 0.007 < 0.001 < 0.001 0.42 < 0.001
Firmicutes Clostridium 1.86 1.98 1.87 0.041 1.74 2.03 1.98 0.061 0.02 < 0.001 0.38 < 0.001 < 0.001
Actinobacteria Bifidobacterium 2.24 2.13 1.45 0.471 2.1 1.93 1.4 0.323 0.82 < 0.001 < 0.001 0.001 < 0.001
Firmicutes Lachnoclostridium 1.51 1.64 1.45 0.052 1.49 1.56 1.59 0.026 0.10 < 0.001 < 0.001 0.002 < 0.001
Firmicutes Clostridia 1.38 1.47 1.33 0.045 1.27 1.42 1.5 0.053 0.19 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Ruminococcus 1.38 1.35 1.29 0.071 1.05 1.31 1.27 0.07 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001
Actinobacteria Olsenella 1.1 1.16 1.4 0.137 1.25 1.15 1.16 0.114 0.38 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Clostridiales 1.14 1.16 1.07 0.036 1.1 1.13 1.2 0.044 0.41 0.09 < 0.001 < 0.001 < 0.001
Bacteroidetes Prevotellaceae 0.89 0.96 1.11 0.069 1.11 1.05 0.88 0.059 0.01 < 0.001 < 0.001 < 0.001 < 0.001
Bacteria-metatranscriptomics
Bacteroidetes Prevotella 17.65 16.96 18.74 0.749 16.1 16.42 14.9 0.559 0.26 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Lachnospiraceae 3.81 4.16 3.9 0.144 4.39 4.05 4.62 0.15 0.20 < 0.001 < 0.001 < 0.001 < 0.001
Bacteria 3.74 3.68 3.61 0.07 3.63 3.58 3.77 0.056 0.71 < 0.001 0.56 0.001 < 0.001
Fibrobacteres Fibrobacter 3.77 3.65 3.92 0.425 3.15 2.8 3.22 0.269 0.21 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Ruminococcus 3.47 3.71 3.05 0.218 2.59 2.83 2.86 0.145 0.16 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Butyrivibrio 3.17 2.98 2.77 0.218 3 2.95 2.9 0.195 0.52 0.002 < 0.001 < 0.001 < 0.001
Firmicutes Lachnoclostridium 1.97 2.13 1.99 0.068 2.28 2.05 2.53 0.1 0.005 < 0.001 < 0.001 < 0.001 < 0.001
Bacteroidetes Bacteroides 2.01 1.87 1.96 0.074 2.04 1.92 1.74 0.069 0.98 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Clostridium 1.24 1.47 1.71 0.199 2.5 1.56 1.96 0.259 < 0.001 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Eubacterium 1.51 1.72 1.6 0.073 2.06 1.68 1.71 0.111 0.10 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Clostridia 1.54 1.54 1.41 0.065 1.61 1.49 1.79 0.075 0.51 < 0.001 0.65 < 0.001 < 0.001
Firmicutes Clostridiales 1.34 1.36 1.28 0.05 1.4 1.36 1.6 0.06 0.42 < 0.001 < 0.001 < 0.001 < 0.001
Actinobacteria Olsenella 1.12 1.15 1.49 0.116 0.94 1.27 1.53 0.129 0.34 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Faecalibacterium 1.24 1.24 1.16 0.036 1.16 1.21 1.38 0.04 0.92 < 0.001 < 0.001 < 0.001 < 0.001
Actinobacteria Bifidobacterium 1.34 1.57 0.77 0.407 1.72 0.78 1.11 0.17 0.45 < 0.001 < 0.001 < 0.001 < 0.001
Proteobacteria Proteobacteria 1.3 1.2 1.19 0.045 1.1 1.21 1.1 0.056 0.003 < 0.001 < 0.001 < 0.001 < 0.001
Bacteroidetes Prevotellaceae 1.12 1.24 1.4 0.11 1.12 1.17 1.04 0.06 0.65 < 0.001 < 0.001 < 0.001 < 0.001
Firmicutes Megasphaera 0.27 0.3 1.08 0.29 0.23 3.83 0.3 1.302 0.92 < 0.001 < 0.001 < 0.001 < 0.001