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Table 1 PERMANOVA analysis for 16S rRNA archaeal amplicon sequencing data. Treatment: control and 3-nitrooxypropanol (3-NOP); week: W4, W8, and W12. (.) P < 0.1; (*) P < 0.05; (**) P < 0.01; (***) P < 0.001; NS, not significant

From: The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows

DNA solid RNA solid
  R2 P value    R2 P value  
Treatment 0.219 0.001 *** Treatment 0.231 0.002 **
Week 0.254 0.001 *** Week 0.247 0.001 ***
Treatment: Week 0.030 0.30 NS Treatment: Week 0.021 0.36 NS
DNA liquid RNA liquid
Treatment 0.144 0.16 NS Treatment 0.157 0.004 **
Week 0.187 0.05 . NS Week 0.207 0.01 *
Treatment: Week 0.010 0.68 NS Treatment: Week 0.010 0.66 NS
Liquid vs solid in DNA Liquid vs solid in RNA
Phase 0.152 0.004 ** Phase 0.237 0.001 ***
DNA vs RNA in solid DNA vs RNA in liquid
Nucleic acid 0.202 0.001 *** Nucleic acid 0.182 0.001 ***