Skip to main content
Fig. 1 | Microbiome

Fig. 1

From: The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows

Fig. 1

Rumen archaeal diversity and composition in control and 3-nitrooxypropanol (3-NOP) treated cows at weeks 4, 8, and 12. 16S rRNA archaeal diversity: A species richness, B Shannon diversity, and C comparison of overall community between samples by weighted UniFrac distances in DNA-based and RNA-based 16S rRNA analysis (beta diversity). Archaeal genera: comparison of archaeal composition at genus level for D DNA-based 16S rRNA, E RNA-based 16S rRNA, F metagenomics (metaG), and G metatranscriptomics (metaT). rt-PCR: quantification of selected methanogens at week 8 by real time PCR (rt-PCR) for HMethanosphaera stadtmanae DSM309 (mtaB), IMethanobrevibacter ruminantium M1 (mcrG), JMethanobrevibacter smithii ATCC35061 (mtaB), and K 16S rRNA copy number. PCoA, principal coordinates analysis. NS, no statistical significance in generalized linear mixed model (glmer); *P < 0.05; **P < 0.01; ***P < 0.001. The black circles appearing on the boxplots are the outlier samples

Back to article page

Microbiome

ISSN: 2049-2618

Contact us