Fig. 3
Meta-epigenomic profile of MAGs across all sampling stations. a, Principal coordinate analysis (PCoA) clustering by the 5-mer methylation features of Shipborne Pole-to-Pole Observations (SHIPPO) MAGs based on Kulczynski dissimilarity. Each point represents each species-level MAG in each sample. The black-dashed circles represent family-level clusters of MAGs across samples. The colored-solid lines represent species-level clusters belonging to Pelagibacteraceae across samples. b The maximum methylation ratios of motifs are represented in each MAG at the family level; the highest methylation value among all sample sites is colorized. c Population differentiation versus methylome across sampling stations. For the most prevalent Shipborne Pole-to-Pole Observations (SHIPPO) MAGs, scatterplots show the relationship of 5-mer methylome dissimilarity based on Bray–Curtis and population differentiation by sampling distance. MAG: metagenome-assembled genome (eukaryotic: eukMAG, prokaryotic: proMAG, viral MAG: vMAG, giant viral MAG: gvMAG); FST: fixation index