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Fig. 2 | Microbiome

Fig. 2

From: Strain-level profiling with picodroplet microfluidic cultivation reveals host-specific adaption of honeybee gut symbionts

Fig. 2

Strain-level compositions of honeybee gut shift after droplet-based cultivation. A Schematic of the data analysis workflow. After base quality control, the bacterial species abundance and strain-level genomic variation were estimated using the MIDAS pipeline, and metagenomic binning was performed to restore the metagenome-assembled genomes. B Species-level profiles for the gut sample (GUT) and the picodroplet samples using the Brain Heart Infusion (BHI) or the MRS broth (MRS) after cultivation. C, D Fraction of single-nucleotide variants (SNVs) within core genes in each sample for Lactobacillus (C) and Bifidobacterium (D). E–H Principal coordinate analysis plots based on the pairwise fractions of shared SNVs (Jaccard distance) for the species Lactobacillus melliventris (E), Lactobacillus helsingborgensis (F), Bifidobacterium choladohabitans (G), and Bifidobacterium polysaccharolyticum (H). Dots represent individual samples, color-coded by the medium used for cultivation

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