Fig. 5

The mucin O-glycans-microbiota axis contributes to gut homeostasis. A Weighted UniFrac PCoA plots of colonic mucin fermentation in vitro. B Discriminating taxa between the mucins from diarrheal piglet and healthy control fermentation, as determined by LEfSe analysis (LDA≥2). C Representative images of E. coli k88 adherent to the T84 cells after treatment with mucin O-glycans inhibitor benzyl-α-GalNAc. The mRNA levels of D inflammatory cytokines, E ER-related genes, and F tight junction protein-related genes in the T84 cells after treatment with mucin O-glycans inhibitor benzyl-α-GalNAc. Data are presented as mean ± SE. H, healthy controls; D, diarrheal piglets; CON, control group; ETEC, E. coli k88 group; Ben, E. coli k88+Ben-α-Gal group