Fig. 2

Diarrheal piglets show abnormal mucin O-glycan profile. A PLS-DA of mucin O-glycan profile in the colon. B Sankey flow diagram of O-glycan structures in two groups. The width of the flow corresponds to the relative abundance of structures. Red arrowhead indicates structures altered in piglets. P<0.05. C Heatmap illustrating the altered relative abundance of mucin O-glycans. P<0.1. Data are presented as relative abundance. The relative abundance of DO-glycan chain length and EO-glycan structures in the colon. Data are presented as min to max showing all points. F Pairwise comparisons of differently abundant taxa are shown, with a color-gradient denoting Spearman’s correlation coefficients. O-glycans are related to each taxa by partial Mantel tests. Edge width corresponds to the Mantel’s statistic for the corresponding distance correlations, and edge color denotes the statistical significance. H, healthy controls; D, diarrheal piglets; PLS-DA, partial lease-squares discriminant analysis