Fig. 3

Heatmaps displaying the log ratios of MAGs and virMAGs relative abundance in each condition over their mean relative abundance across samples. A The 50 most abundant viral MAGs are displayed, and the average abundance is calculated on the supernatant samples. Four clusters are highlighted: cluster “1” is exclusively composed of Siphoviridae phages undergoing a mild increase during microaeration; cluster “2” is characterised by a marked increase during overload as well as a decrease in pH; cluster “3” is similar to cluster “2”, but the variations are less evident; cluster “4” is composed of phages sharply decreasing during overload and increasing when exposed to basic pH. B The 50 most abundant prokaryotic MAGs are displayed, and the average relative abundance is calculated across pellet samples. Coherent behaviours in terms of response to conditions are depicted as clusters: cluster “A” comprises MAGs increasing in relative abundance when exposed to basic pH and negatively affected by microaeration; cluster “B” is composed of MAGs with slightly increased average relative abundance under microaeration; in cluster “C” and cluster “D”, respectively, a sharp decrease and increase during overload treatment are evidenced. C The summary table contains some functionally relevant species discussed in the main text