Fig. 5
From: Developing whole cell standards for the microbiome field
Comparison of selected DNA extraction kits when using mock communities of different microbial compositions. Kits selected were Kit 2, Kit 6, and Kit 7. Data is based on shotgun sequencing of DNA analysed using MetaPhlAn3. Mock communities used were NIBSC WC-Gut RR, ZymoBIOMICS Microbial Community Standards (ZYMO), 20 Strain Even Mix Whole Cell Material (ATCC-MSA 2002), and 10 Strain Even Mix Whole Cell Material (ATCC-MSA 2003). A Sensitivity. B Similarity in composition with the actual composition. C False-positive relative abundance (FPRA). D Relative abundance of gram-positive and gram-negative bacteria in the samples. The results are based on the average of three replicates