Table 1 List of genes and primer sequence for quantitative real-time PCR analysis
From: Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
Gene | Primer sequences (5′ to 3′) | Accession No. |
|---|---|---|
β-actin | f-TTGTTGACAATGGCTCCGGT | NM_205518.1 |
r-TCTGGGCTTCATCACCAACG | ||
IL-1β | f-ACCTACAAGCTAAGTGGGCG | NM_204524.1 |
r-ATACCTCCACCCCGACAAGG | ||
TNF-α | f-CAGATGGGAAGGGAATGAAC | AY765397.1 |
r-CACACGACAGCCAAGTCAAC | ||
IFN-γ | f-CTCGCAACCTTCACCTCACCATC | NM_205149.1 |
r-CAGGAACCAGGCACGAGCTTG | ||
IL-6 | f-CTCCTCGCCAATCTGAAGTC | NM_204628.1 |
r-AGGCACTGAAACTCCTGGTCT | ||
IL-12 | f-ATTACTTTCCTTTGCTGCCCTTC | NM_213571.1 |
r-CTGGTGTCTCATCGTTCCACTC | ||
TLR4 | f-TGAAAGAGCTGGTGGAACCC | NM_001030693.1 |
r-CCAGGACCGAGCAATGTCAA | ||
MyD88 | f-AGGATGGTGGTCGTCATTTC | NM_001030962.2 |
r-TTGGTGCAAGGATTGGTGTA | ||
NF-κB | f-CTACTGATTGCTGCTGGAGTTG | M86930.1 |
r-CTGCTATGTGAAGAGGCGTTGT | ||
IL-4 | f-AGCCAGCACTGCCACAAGAAC | NM_001007079.1 |
r-CGTGGGACATGGTGCCTTGAG | ||
IL-10 | f-CAGCACCAGTCATCAGCAGAGC | NM_001004414.2 |
r-GCAGGTGAAGAAGCGGTGACAG | ||
TGF-β | f-ATGTGTTCCGCTTTAACGTGTC | NM_205454.1 |
r-GCTGCTTTGCTATATGCTCATC | ||
MUC2 | f-AATGCTGAGTTCTTGCCTAA | XM_001234581.3 |
r-TGTTGCAGTTCATATCCTGGT | ||
Occludin | f-CGCAGATGTCCAGCGGTTACT | NM_205128.1 |
r-CAGAGCAGGATGACGATGAGGAA | ||
ZO-1 | f-CCACTGCCTACACCACCATCTC | XM_015278975.1 |
r-CGTGTCACTGGGGTCCTTCAT | ||
Claudin-1 | f-GCATGGAGGATGACCAGGTGA | NM_001013611.2 |
r-GAGCCACTCTGTTGCCATACCAT | ||
Caspase-3 | f-TCCACCGAGATACCGGACTG | NM_204725.1 |
r-ACAAAACTGCTTCGCTTGCT | ||
Bax | f-GGGGTACGTCAATGTGGTCA | XM_015274882.1 |
r-AGGAAGGCGGTGGGATAATG | ||
Bak | f-GTTCCGGAGCTACACCTTCT | NM_001030920.1 |
r-GTACCGCTTGTTGATGTCGT | ||
Bcl-2 | f-ATGACCGAGTACCTGAACCG | NM_205339.2 |
r-CAAGAGTGATGCAAGCTCCC | ||
P53 | f-GCTGAACCCCGACAATGAGA | NM_205264.1 |
r-TTTGCAGCAGTTTCTTCCCG |