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Table 1 List of genes and primer sequence for quantitative real-time PCR analysis

From: Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation

Gene Primer sequences (5′ to 3′) Accession No.
β-actin f-TTGTTGACAATGGCTCCGGT NM_205518.1
r-TCTGGGCTTCATCACCAACG
IL-1β f-ACCTACAAGCTAAGTGGGCG NM_204524.1
r-ATACCTCCACCCCGACAAGG
TNF-α f-CAGATGGGAAGGGAATGAAC AY765397.1
r-CACACGACAGCCAAGTCAAC
IFN-γ f-CTCGCAACCTTCACCTCACCATC NM_205149.1
r-CAGGAACCAGGCACGAGCTTG
IL-6 f-CTCCTCGCCAATCTGAAGTC NM_204628.1
r-AGGCACTGAAACTCCTGGTCT
IL-12 f-ATTACTTTCCTTTGCTGCCCTTC NM_213571.1
r-CTGGTGTCTCATCGTTCCACTC
TLR4 f-TGAAAGAGCTGGTGGAACCC NM_001030693.1
r-CCAGGACCGAGCAATGTCAA
MyD88 f-AGGATGGTGGTCGTCATTTC NM_001030962.2
r-TTGGTGCAAGGATTGGTGTA
NF-κB f-CTACTGATTGCTGCTGGAGTTG M86930.1
r-CTGCTATGTGAAGAGGCGTTGT
IL-4 f-AGCCAGCACTGCCACAAGAAC NM_001007079.1
r-CGTGGGACATGGTGCCTTGAG
IL-10 f-CAGCACCAGTCATCAGCAGAGC NM_001004414.2
r-GCAGGTGAAGAAGCGGTGACAG
TGF-β f-ATGTGTTCCGCTTTAACGTGTC NM_205454.1
r-GCTGCTTTGCTATATGCTCATC
MUC2 f-AATGCTGAGTTCTTGCCTAA XM_001234581.3
r-TGTTGCAGTTCATATCCTGGT
Occludin f-CGCAGATGTCCAGCGGTTACT NM_205128.1
r-CAGAGCAGGATGACGATGAGGAA
ZO-1 f-CCACTGCCTACACCACCATCTC XM_015278975.1
r-CGTGTCACTGGGGTCCTTCAT
Claudin-1 f-GCATGGAGGATGACCAGGTGA NM_001013611.2
r-GAGCCACTCTGTTGCCATACCAT
Caspase-3 f-TCCACCGAGATACCGGACTG NM_204725.1
r-ACAAAACTGCTTCGCTTGCT
Bax f-GGGGTACGTCAATGTGGTCA XM_015274882.1
r-AGGAAGGCGGTGGGATAATG
Bak f-GTTCCGGAGCTACACCTTCT NM_001030920.1
r-GTACCGCTTGTTGATGTCGT
Bcl-2 f-ATGACCGAGTACCTGAACCG NM_205339.2
r-CAAGAGTGATGCAAGCTCCC
P53 f-GCTGAACCCCGACAATGAGA NM_205264.1
r-TTTGCAGCAGTTTCTTCCCG