Fig. 7

A. muciniphila downregulate the colitis predisposition in susceptible mice through induction of suppressive RORγt⁺ Treg cells. Colitis was induced by transferring CD4⁺ T cells into Rag1-deficient (Rag1^−/−) mice colonized with A. muciniphila or BHI medium. a T-cell transfer strategy. b Body weight change. Thin lines represent the mean data from a group of 6 mice, and bold lines represent the mean ± SEM of all groups of mice colonized either with A. muciniphila or BHI medium. c Representative pictures of colon gross appearance. d Colon length. e Representative microscopic pictures of H&E staining (100× and 200× magnification). f Histology score. g Representative flow cytometric analysis of colonic RORγt⁺ Treg cells between (Rag1^−/− + Akk) and (Rag1^−/− + BHI) mice. h Statistics results of Foxp3⁽⁺⁾ RORγt⁽⁻⁾ T cells frequency. i Statistics results of Foxp3⁽⁻⁾ RORγt⁽⁺⁾ T cells frequency. j Statistics results of Foxp3⁽⁺⁾ RORγt⁽⁺⁾ T cells frequency. a–f n = 6 mice per group, g–j n = 5 mice per group, mean values ± SEM are presented, p-values were calculated using unpaired T-test, *p < 0.05, **p < 0.01, ***p < 0.001. Data are pooled from three independent experiments