Skip to main content
Fig. 6 | Microbiome

Fig. 6

From: TLR4 regulates RORγt+ regulatory T-cell responses and susceptibility to colon inflammation through interaction with Akkermansia muciniphila

Fig. 6

A. muciniphila administration suppresses colon inflammation both in WT and TLR4−/− mice through regulating T-cell-associated immune responses. After incubation with A. muciniphila or BHI for 21 days, mice were consistently given oral administration of 2.5% DSS for 7 days followed by normal water drinking for a further 7 days. a Body weight change. b Survival. c DAI score. d Representative pictures of colon gross appearance. e Colon length. f Representative microscopic pictures of H&E staining (40× and 200× magnification). g Histology score. h Representative flow cytometric analysis of colonic and spleen RORγt+ Treg cells among WT Akk, TLR4−/− Akk, WT BHI, TLR4−/− BHI groups. Numbers in outlined areas indicate percent cells in each gated area. i Statistical analysis of CD4(+) Foxp3(+) RORγt(+) T cells frequency in colonic LP among groups. j Statistical analysis of CD4(+) Foxp3(+) RORγt(+) T cells frequency in spleen among groups. ag n = 10 mice per group, hj n = 6 mice per group, mean values ± SEM are presented, p-values were calculated using two-way analysis of ANOVA test, *p < 0.05, **p < 0.01, ***p < 0.001. Data are pooled from three independent experiments

Back to article page