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Fig. 6 | Microbiome

Fig. 6

From: Soil-derived bacteria endow Camellia weevil with more ability to resist plant chemical defense

Fig. 6

Experimental analysis showing that Acinetobacter sp. AS23 from soil mediates the adaptation of CWs to tea saponin toxicity. To further investigate the saponin-degrading functions of AS23, PBBR-GFP plasmid was transferred into the strain as described by Zhang et al. [52]. a Schematic showing the treatment of CW larvae in the experiment. Antibiotic treatment: gentamicin sulfate, tetracyclines, and rifampin. CK treatment: sterile water. SS treatment: larvae near pupation were raised in sterilized soil. US treatment: larvae near pupation were raised in unsterilized soil. SSA treatment: larvae near pupation were raised in sterilized soil mixed with fluorescently labeled Acinetobacter sp. AS23 cells. b CW adults were released on C. oleifera trees, and the fruits were regularly collected to evaluate larvae development. c, d Green fluorescent imaging of AS23 cells (Acinetobacter sp. AS23) in the guts of collected larvae and fluorescence imaging of guts. e, f Fluorescent imaging of guts uninfected with fluorescently labeled Acinetobacter sp. strain 23. g Comparison of larval weights from individuals obtained at different sampling time. h Analysis of larval weight changes from individuals collected at different sampling time. Data were normally distributed, and ANOVA analysis was performed using Prism GraphPad. A p value threshold of significance was identified as p < 0.05

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