Fig. 1
From: Soil-derived bacteria endow Camellia weevil with more ability to resist plant chemical defense
Sample collection information and microbiome diversity and species composition analysis from fruit, soil, and weevil gut microbiomes. a Geographic distribution of sampling locations. Three plantations (QT: Qiangtian, QZ: Quzhou, and JD: Jiande) chosen for experiment. b Images of C. oleifera. c Images of C. oleifera clones’ fruits with saponin content increase. Five C. oleifera clones (CL 3: Changlin 3, CL 40: Changlin 40, CL 53: Changlin 53, CL 55: Changlin 55, and CL 166: Changlin 166) selected for the study. d Unconstrained PCoA ordination based on Bray–Curtis distances showing that three community types are completely disparate from each other (p = 0.001, PERMANOVA test and Anosim test). e Top 10 of the most abundant discriminatory bacterial genera were identified by applying random-forest classification of ASV relative abundances among sample types. Biomarker taxa are ranked in descending order of importance based on the classification model. f Ternary plots of amplicon sequence variants (ASVs) identified in all samples and their relative abundances. Each point represents a single ASV. Only ASVs found in at least 10% of the samples are included in the figure. The size of each symbol indicates the relative abundance (weighted average) of the ASVs, and its color indicates the sample type. Green depicts ASVs found in > 50% in gut samples, red depicts ASVs found in > 50% in fruit samples, blue depicts ASVs found in > 50% of soil samples, and grey symbols indicate general ASVs found in all sample types. The position of each point reflects its contribution to the total relative abundances of the indicated sample types. g Top 10 of the most abundant ASVs are re-colored from fruit, soil, and weevil gut microbiomes. Only ASVs found in at least 10% of the samples are included in the figure. The size of each symbol indicates the relative abundance (weighted average) of the ASV, and its color indicates top 10 of the most abundant ASVs. The position of each point reflects its contribution to the total relative abundances of the indicated sample types