Fig. 8

Local DNA methylation changes persist from F1 to F2 generation. a Snapshot of Integrative Genomics Viewer Windows which displays CpG methylation data from scaffold 1792:239,000–242,000 for oysters sampled at day 10 of F1 and F2 generation (F1-D10, F2-D10, respectively) and at day 120 just before disease induction of F1 and F2 generation (F1-D120H0, F2-D120H0, respectively). Each bar indicates the level of methylation at a CpG position (x-axis) on a scale from 0 to 100% (y-axis). The three biological replicates for each time point are represented. The hypomethylation displayed in the F1 juvenile oysters (F1-D120H0) in ME exposed (pink color) compared to control oysters (blue color) was detected with the DMRseq software and was inherited from F1 to F2 generation in larvae (F2-D10) and juveniles (F2-D120H0). b Box plots representing the distribution of the level of methylation (mean of three biological replicates) for each CpG position present in the selected region. *Indicates a p-val < 0.05 of a Mann-Whitney analysis comparing the ME exposed and control oysters for time point