Fig. 4

Healthy and UC VLP administration increases viral abundance and VBR in UC-HMA mice. Data shown is from experiment “B” (trial #1). A Viral abundance was determined from mouse fecal pellets using epifluorescence microscopy and compared to B bacterial abundances obtained by flow cytometry after staining with SybrGREEN I to obtain C VBRs. D Mean total viral abundance and mean total VBR post-VLP gavage were compared between treatment groups after the first dose of VLPs or PBS was given to mice. A–C Significance was assessed using a repeated measure two-way ANOVA and Dunnett’s multiple comparisons test (*p ≤ 0.05, **p≤ 0.01) and using a Geisser-Greenhouse correction. Red and blue asterisks indicate significant differences between the PBS control and HMA mice given UC VLPs and healthy VLPs, respectively. Dots represent abundance or VBR of pooled mouse fecal samples at a single sampling point. At each sampling point, mouse fecal samples in each cage were pooled from 2 mice (n=3 cages per group, 6 mice per group). Error bars, SE. UC bac, UC-HMA mice