Fig. 2

Maximum likelihood (LG + R10, general matrix and FreeRate model with 10 categories for amino acid substitution; 1000 ultrafast bootstraps) phylogeny for the CPR radiation based on 38 concatenated SCGs (Supplementary Table 2). The lake origin of each freshwater MAG obtained from this study is marked by a dot with a different color at the end of the tips. CPR classes are shown with distinct colors in the inner circle. Following annotations starting from the inner circles represent (1) the isolation source of the genome; (2) the estimated genome size; (3) the trophic state of the lake; (4) the relative abundance of each MAG in metagenomic read recruitment expressed as coverage per Gb of metagenome; (5) the GRiD values for estimation of bacterial replication rates. Probe targets for CARD-FISH visualization are indicated by different colors and the name of probes marked with light blue