Fig. 2

Changes in the fecal metabolomic profiles of the diarrheic calves. a The fecal metabolomic profiles were clustered using PLS-DA. The metabolomic profiles for the H_1, H_2, D_1 and D_2 groups are shown in the same colors. Data are presented as the mean ± SEM. P-values were determined using the nonparametric Kruskal-Wallis test. b Relative abundances of metabolites were clustered using a UPGMA dendrogram and shown in a heatmap. Color indicates the relative abundances of the metabolite in the group of samples; the corresponding relationship between the color gradient and the value is shown in the gradient color block. The metabolite variation is shown using Z_Score. UDCA is in red. The concentrations of fecal UDCA (c), β-UDCA (d), UCA (e), and MCA (f) are displayed as box and dot plots. Data are presented as the mean ± SEM. P-values were determined using the nonparametric Kruskal-Wallis test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001