Fig. 2

a Relative abundance of bacterial genera in individual adult mosquitoes sampled from conventionally maintained populations of each of the donor species used in the study (i.e., Ae. aegypti and Cx. quinquefasciatus). Adults were provided 10% sucrose in water (wt/vol) ad libitum prior to being sampled 3–4 day postemergence. Each bar represents an individual mosquito. Low abundance genera (< 1%) are represented by the ‘Other’ category. b and c Alpha and beta diversity of Ae. aegypti (Aa) and Cx. quinquefasciatus (Cq) donor individuals. Panel b shows the difference in alpha diversity between Ae. aegypti and Cx. quinquefasciatus donor individuals as measured by Shannon’s H index (left) and ASV richness (right). Box-and-whisker plots show high, low, and median values, with lower and upper edges of each box denoting first and third quartiles, respectively. Asterisks (***) indicate significant differences between Ae. aegypti and Cx. quinquefasciatus donor individuals (Kruskal-Wallis test, P < 0.05). Panel c shows the difference in beta diversity between Ae. aegypti and Cx. quinquefasciatus donor individuals. Principal coordinates analysis using the Bray-Curtis dissimilarity index identified significant clustering by donor species (PERMANOVA, P = 0.001)