Fig. 5

Association between eukaryotic and prokaryotic MAGs. At the top, scatter plots showing coverage of pairs of MAGs. Coverage of eukaryotes is on the vertical axis, and coverage of a prokaryotic MAG is on the horizontal axis. Each point shows coverage values in one sample. Colour of points indicates whether the point is a polar (blue) or non-polar (red) sample. Linear regression was performed with an ordinary least squares method for each pair, and regression line is shown in dashed grey. We retained pairs with R² ≥ 0.7 and p ≤ 0.05 after highly influential points (outliers) were removed. Only prasinophytes are shown here; the remainder are available in Additional file 8. The lower plot shows GO terms which appear enriched in one of the associated pairs, NP2_2E and NP3_22P, a Bathycoccus and Erythrobacter, respectively. Enrichment was assessed via Fisher’s exact test detailed in the “Methods” section. Each circle represents an enriched term, and size is scaled based on the odds ratio. Left peach terms are those in the eukaryotic MAG, and right pink terms are those in the associated prokaryotic MAGs. Terms from the cellular component and molecular function GO terms are shown, divided vertically