Fig. 5

SameStr-based unsupervised strain sharing networks identify potentially mislabeled samples. Shared strain profiles were visualized as unsupervised networks with individual samples as nodes and shared strain numbers as edges. A These networks connect samples from Louis et al. [38] by individual, with the exception of two samples (AS64_24 and AS66_24) that appear to be mixed up. B In a case of multiple rCDI patients treated with FMT from the same donor [15], shared strains were detected between pre- (blue) and post-FMT (yellow) patient samples, as well as between post-FMT and donor (green) samples and among post-FMT samples. Pre-FMT samples did not share strains with donor samples, with the exception of FMT15, which shares (> 15) strains with all three donor samples and exhibits ɑ/β-diversity compositions that are comparable to other post-FMT samples (data not shown). As this sample was collected on the day of the FMT procedure, FMT15 could in fact represent a post-FMT sample that was accidentally mislabeled as a pre-FMT sample (Smillie, personal communication)