Fig. 6

Immunologic changes correlate with gut microbial alterations. Heatmaps show latent correlation matrices between abundances of amplicon sequence variants (ASVs) detected in stool samples and all immune parameters analyzed in colon (A) and spleen (B) of mice 21 days after inoculation with AdLib and CalRes human gut microbiota. Immune parameters are expressed as frequencies, i.e., percent of parent, except those labeled # which were quantified as absolute cell counts. Heatmaps were ordered according to rows and columns first principal components to highlight cross-correlation structures. Asterisks indicate variables that were selected in L1-penalized sparse canonical correlation analysis (CCA). Circular chord plots display latent correlation between frequencies of manually defined immune subsets and L1-selected ASVs including the top ten taxa that either positively (upper) or negatively (lower) associate with the immunological dataset. Blue to red color scale in heatmaps and chords indicates negative and positive correlation values. Color of row-legend bar and species labels denotes the phylum level. Colors of column legend bars indicate parental lineage and differentiation level (antigen-experience) of lymphocyte subsets, respectively. Boxplot insets show how experimental groups as a latent variable are explained by the canonical covariate