Fig. 2

Colonization with CalRes-associated gut microbiota alters body fat and glucose clearance. Metabolic analysis of germ-free (GF) mice and mice inoculated with AdLib and CalRes human gut microbiota. A–C Spleen (A), epigonadal white adipose tissue (eWAT) (B), and liver (C) weights from GF and colonized mice. D Feces weight was measured using bomb calorimetry in GF and colonized mice. E Caecum weights from GF and colonized mice were analyzed with and without fecal contents. F Fasting male adult GF or colonized mice maintained on normal chow diet were challenged with oral glucose and blood was sampled for glucose at times indicated. G Area under the OGTT glucose-time curve (AUC). * P < 0.05, ** P < 0.01, **** P < 0.0001 as determined using ANOVA with Bonferonni’s post-test correction for multiple comparisons; error bars = SEM; ns = not significant