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Fig. 1 | Microbiome

Fig. 1

From: Effects of caloric restriction on the gut microbiome are linked with immune senescence

Fig. 1

Caloric restriction changes the gut microbiome composition in humanized gnotobiotic mice. A Number of observed amplicon sequence variants (ASVs) and Shannon index indicate microbial richness and diversity for 3 weeks following colonization. P-values are given for repeated measures non-parametric ANOVA-type testing for differences between groups and time points (n = 104 stool samples across 23 mice and 5 time points). B Principal coordinates of Bray-Curtis dissimilarity between stool samples. Color indicates groups. Numeric labels denote the 5 time points. Ellipses enclose group masses (99% CI) for day 21. P-values are given for adonis permutational analysis of variance testing for group × time differences. C Heatmap representation of day 21 differentially abundant ASVs (n = 20). ASVs are shown at the most specific assigned taxonomy with FDR-adjusted P-values < 0.01 in DESeq2 two-sided Wald test. Bubbles indicate log-fold change (LFC) between the two groups. Heatmap colors indicate relative abundances as variance-stabilizing (log2) transformed counts ranging from 4 (dark blue, corresponding to 0 raw counts) to 14 (yellow, corresponding to 14K raw counts). Color of bubbles and species labels denote phylum. Bubble sizes are proportional to − log10 FDR-adjusted P-values. Dendrogram leaf numbers indicate individual housing cages

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