Fig. 5

Fecal microbiota structure pre- and post-FMT. A PCoA showing clustering of fecal microbiota samples from young (blue), old (white), and aged (orange) mice pre-antibiotic treatment (Pre-Abx) overlaid with species driving the clustering. B Significantly enriched species in aged mice (orange bars, n = 8) vs. young mice (blue bars, n = 7) pre-antibiotic treatment (Pre-Abx); the results are displayed as the mean difference in centered log ratio, CLR, and SE. C PCoA (Bray–Curtis) depicting clustering of all mice from all age and treatment groups pre- (yellow) and post- (green) antibiotic treatment, post-FMT (magenta), and at the end of the experiment (“End,” gray). D PCoA (Bray–Curtis) depicting clustering of all mice from all age and treatment groups, colored by age group, young in blue markers, old in white/black markers, and aged in orange markers (see also Fig. S2)