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Fig. 7 | Microbiome

Fig. 7

From: Spatial organization of the kelp microbiome at micron scales

Fig. 7

Unipolar labeling of adherent Alphaproteobacteria by wheat germ agglutinin. Wheat germ agglutinin was used to stain N-acetylglucosamine and N-acetylmuramic acid residues. Staining was observed on Alphaproteobacteria rods at only one end, showing apparent polarity with respect to the cells. A Hybridization of a kelp sample with probes for Alphaproteobacteria (cyan) and Granulosicoccus (magenta). B Signal from fluorophore-labeled WGA (red) in the same field of view as (A). C Merged image of A and B showing that WGA staining was observed in cells hybridizing with the Granulosicoccus and Alphaproteobacteria probes. WGA staining was detected surrounding the Granulosicoccus cells while the stained Alphaproteobacteria showed fluorescence at only one end of the cell. WGA signal associated with Alphaproteobacteria was brighter and more localized than that associated with Granulosicoccus. D and E Representative images of FISH on whole-mount samples from kelp blades collected in different months from Tatoosh Island. F Cross-sectional image showing Alphaproteobacteria rods with the polar polysaccharide end adjacent to the kelp surface

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