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Fig. 8 | Microbiome

Fig. 8

From: The phyllosphere microbiome shifts toward combating melanose pathogen

Fig. 8

Suppressive activities of selected microbes from the changed component of the phyllosphere microbiome against the melanose pathogen D. citri. a The antagonistic activities in dual culture assays. A mycelial plug of D. citri was placed in the center of the medium, and tested isolates were streaked on four ends. Plates inoculated with D. citri only served as control. b Effect of microbial metabolites on D. citri spore germination in vitro. The D. citri spore suspension spread on water agar medium supplemented with 10% cell-free supernatant of tested isolates. The spore suspension spread on water agar medium supplemented with 10% 1/2 TSB medium served as control. c Effect of iron nutrition on antagonistic activity of Sphingomonas asv20 against D. citri in dual culture assays. d Disease suppression of melanose in the phyllosphere treated with Sphingomonas asv20, Pantoea asv90, Methylobacterium asv41, and Mucilaginibacter asv102 in glasshouse experiments. Non-treated seedlings were used as controls. Disease index and disease incidence were scored at 10 days after D. citri inoculation. Different letters indicate statistically significant differences between treatments as determined by one-way ANOVA and LSD test (P < 0.05). e Pictures showing citrus seedling leaves treated/untreated with Sphingomonas asv20 at 10 days after D. citri inoculation

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