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Fig. 1 | Microbiome

Fig. 1

From: A mouse model of occult intestinal colonization demonstrating antibiotic-induced outgrowth of carbapenem-resistant Enterobacteriaceae

Fig. 1

K. pneumoniae occultly colonizes the gastrointestinal tract for up to 4 weeks, with outgrowth following receipt of an antibiotic cocktail. a Study designs to test inoculation size, occult location, and time until antibiotic. (Top) Mice were orally gavaged with 103, 104, or 105 CFU of K. pneumoniae (Kp) or the PBS vehicle control at day 0 (triangle). An antibiotic cocktail was given from day 7 to day 21. Days of stool collection are marked by circles. Mice were euthanized at day 21, and cecal contents collected. (Middle) Mice were orally gavaged with 105 CFU of K. pneumoniae, and tissues were plated at day 7. (Bottom) Mice were orally gavaged with 105 CFU of K. pneumoniae, and different groups of mice were given antibiotic for 2 weeks starting at t = 1, 2, 4, or 8 weeks post-gavage. Stool and cecal contents were collected at (t+2)th week. b CFU of K. pneumoniae in stool and cecal contents (cec). N=6. The dotted line marks the limit of detection for CFU. c CFU of K. pneumoniae in stool, cecal, and small intestinal (SI) contents and the mesenteric lymph nodes (MLN), liver, and blood plasma. N=10. d CFU of K. pneumoniae at (t+2)th weeks in stool and cecal content. N=6. For b, Fisher’s exact test with Hommel correction for multiple testing was used to compare numbers of mice showing outgrowth versus no-outgrowth in inoculated mice to those in the PBS control group. Outgrowth is defined as CFU > 0. *p value < 0.05, **p value < 0.01

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