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Fig. 9 | Microbiome

Fig. 9

From: Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions

Fig. 9

Development and validation of the C. minuta enrichment and sorting strategy. Original fecal sample was spiked with 0.1% or 0.01% C. minuta DSM 22607 (A). Bacteria considered as live after staining with the LIVE/DEAD™ BacLight™ Bacterial Viability Kit were selected (B) and among them only events presenting FSC/SSC values similar to those of C. minuta DSM 22607 (as determined in panel D) were considered (C, black circle). Bacteria stained with anti-C. minuta antibodies were then selected from these regions and directly sorted on cultivation plates (E and F, antibodies-stained frequencies relate to total events). Colonies circled in green tested positive for C. minuta-specific PCR. These experiments were performed once for each spiked dilution

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