Fig. 9From: Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditionsDevelopment and validation of the C. minuta enrichment and sorting strategy. Original fecal sample was spiked with 0.1% or 0.01% C. minuta DSM 22607 (A). Bacteria considered as live after staining with the LIVE/DEAD™ BacLight™ Bacterial Viability Kit were selected (B) and among them only events presenting FSC/SSC values similar to those of C. minuta DSM 22607 (as determined in panel D) were considered (C, black circle). Bacteria stained with anti-C. minuta antibodies were then selected from these regions and directly sorted on cultivation plates (E and F, antibodies-stained frequencies relate to total events). Colonies circled in green tested positive for C. minuta-specific PCR. These experiments were performed once for each spiked dilutionBack to article page