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Fig. 6 | Microbiome

Fig. 6

From: Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions

Fig. 6

Enrichment rates of specific phylogenetic groups in the populations sorted after staining with the polyclonal antibodies directed against F. prausnitzii ATCC 27766 + ATCC 277668 (A), F. prausnitzii A2-165 (B), C. minuta DSM 22607 (C), and in bacterial population presenting auto-fluorescence in the 450/50 nm channel after excitation with the 405 nm laser (D). Rates were calculated by dividing normalized numbers of sequences affiliated to each zOTU in the sorted fractions (dark gray) by normalized numbers of sequences affiliated to the same zOTU in the raw material (light gray). Only groups with a least 0.05% affiliated sequences in the 16S rRNA gene amplicon repertoire of pooled fecal samples and for which enrichment rate is ≥ 1 were considered, and a maximum of 15 most enriched groups are shown for each antibody. These experiments were performed once for F. prausnitzii and autofluorescent bacteria (A, B, and D), and once for C. minuta (C)

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Microbiome

ISSN: 2049-2618

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