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Fig. 5 | Microbiome

Fig. 5

From: Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions

Fig. 5

Selective enrichment and 16S rRNA gene amplicon analysis of bacterial populations sorted after staining of pooled fecal samples HV2 + HV4 with polyclonal antibodies directed against F. prausnitzii ATCC 27766 + 27768 and F. prausnitzii A2-165 (A), and with polyclonal antibodies directed against C. minuta DSM 22607 (B). Bacteria presenting auto-fluorescence in the 450/50 nm channel after excitation with the 405 nm laser were also sorted and analyzed by sequencing (A). One million bacteria were sorted for each population to be sequenced, and a 100% identity threshold corresponding to the zero radius OTU (zOTU) definition was used to delineate OTUs [24]. These experiments were performed once for F. prausnitzii (A) and once for C. minuta (B)

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Microbiome

ISSN: 2049-2618

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