Skip to main content
Fig. 5 | Microbiome

Fig. 5

From: Gut microbiota–derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation

Fig. 5

The mediation of IL-35+ B cells by IAA and LPS requires PXR and TLR4. a Percentages of CD19+p35+ and CD19+p35+Ebi3+ cells in the spleens of WT, PXR KO, TLR4 KO, CAR KO, or AHR KO mice with or without exposure to LPS + IAA. b Absolute cell numbers of CD19+p35+ and CD19+p35+Ebi3+ cells in the spleens of WT, PXR KO, TLR4 KO, CAR KO, and AHR KO mice treated with or without LPS + IAA. c Absolute numbers of CD19+p35+ and CD19+p35+Ebi3+ cells in the PPs of WT, PXR KO, TLR4 KO, CAR KO, and AHR KO mice treated with or without LPS + IAA. d ELISA of IL-35 in the spleens, PPs, and sera of WT, PXR KO, AHR KO, CAR KO, and TLR4 KO treated mice with or without LPS + IAA. e Absolute numbers of CD19+p35+Ebi3+ cells in the spleens and PPs of WT, PXR KO, and TLR4 KO mice infused with or without Lactobacillus or LactobacillusΔiaaM (ΔLac). f ELISA of IL-35 in the spleens, PPs, and sera of WT, PXR KO, and TLR4 KO mice infused with or without Lactobacillus or LactobacillusΔiaaM (ΔLac). g IAA levels in the spleens, PPs, and sera of WT, PXR KO, and TLR4 KO mice injected with or without Lactobacillus or LactobacillusΔiaaM (ΔLac). The data in each panel were from three independent experiments. Student’s t test in all panels, mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, NS, no significance

Back to article page